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Home > Product > ELISA Kit > Human Heat Shock 70kDa Protein 1A, HSPA1A ELISA Kit

Human Heat Shock 70kDa Protein 1A, HSPA1A ELISA Kit

Human Heat Shock 70kDa Protein 1A, HSPA1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Heat Shock 70kDa Protein 1A

Code

E3015Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.055ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.

UniProt accession

MASS(Da)

70,052

GeneID

3303;3304

Synonyms

HSPA1A;HSP72;HSPA1;HSX70

Gene names

HSPA1A

Research area

Signal Transduction;Cancer;Neuroscience

Target protein

HSPA1A

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E3015Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.017 2.16 2.12
2.303
12ng/ml 1.404 1.459 1.419
1.513
6ng/ml 0.863 0.894 0.854
0.925
3ng/ml 0.456 0.456 0.417
0.457
1.5ng/ml 0.217 0.256 0.217
0.295
0ng/ml 0.046 0.04 0
0.034

Precision

We measured random samples of E3015Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 8.3 0.4 5.3
Intra-assay 2 18 10.8 0.4 3.8
Intra-assay 3 18 4.2 0.3 6.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E3015Hu

    $320.00

  • 96T

    E3015Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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