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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0208Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ug/ml	2.309	2.172	2.13
	2.035
6ug/ml	1.346	1.316	1.273
	1.285
3ug/ml	0.949	0.945	0.903
	0.941
1.5ug/ml	0.487	0.48	0.437
	0.472
0.75ug/ml	0.238	0.233	0.19
	0.227
0ug/ml	0.052	0.042	0
	0.033

We measured random samples of E0208Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	3.4	0.2	5.7
Intra-assay	2	18	5.5	0.2	3.3
Intra-assay	3	18	2.3	0.2	7.6

> Protocol Booklet

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