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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0191Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.114	2.219	2.167
	2.323
1200ng/L	1.316	1.39	1.338
	1.464
600ng/L	0.903	0.788	0.736
	0.672
300ng/L	0.497	0.461	0.409
	0.425
150ng/L	0.326	0.296	0.244
	0.265
0ng/L	0.06	0.052	0
	0.043

We measured random samples of E0191Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	387.8	23.7	6.1
Intra-assay	2	18	1110.8	33.2	3
Intra-assay	3	18	1036.6	67.5	6.5

> Protocol Booklet

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