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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E2118Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	2.207	2.14	2.094
	2.072
480ng/L	1.689	1.596	1.551
	1.503
240ng/L	0.968	1.046	1.001
	1.125
120ng/L	0.708	0.708	0.663
	0.708
60ng/L	0.426	0.415	0.369
	0.403
0ng/L	0.047	0.045	0
	0.044

We measured random samples of E2118Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	356.1	14.5	4.1
Intra-assay	2	18	175.1	10.9	6.2
Intra-assay	3	18	131.4	9.5	7.2

> Protocol Booklet

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