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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1946Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.395	2.391	2.358
	2.387
800ng/L	1.321	1.304	1.271
	1.287
400ng/L	0.664	0.67	0.637
	0.676
200ng/L	0.517	0.519	0.486
	0.521
100ng/L	0.247	0.234	0.201
	0.22
0ng/L	0.039	0.033	0
	0.028

We measured random samples of E1946Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	590.4	31.5	5.3
Intra-assay	2	18	891.2	31.4	3.5
Intra-assay	3	18	249.3	14.4	5.8

> Protocol Booklet

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