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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0312Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.272	2.154	2.108
	2.037
8ng/ml	1.338	1.331	1.285
	1.324
4ng/ml	0.554	0.567	0.52
	0.579
2ng/ml	0.335	0.329	0.282
	0.323
1ng/ml	0.157	0.163	0.117
	0.169
0ng/ml	0.043	0.046	0
	0.049

We measured random samples of E0312Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	11.6	0.4	3.2
Intra-assay	2	18	3.7	0.2	4.4
Intra-assay	3	18	6.1	0.3	5

> Protocol Booklet

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