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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0111Fi are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.16	2.119	2.044
	2.078
24ng/ml	1.05	1.075	1
	1.101
12ng/ml	0.622	0.647	0.571
	0.671
6ng/ml	0.3	0.306	0.231
	0.313
3ng/ml	0.16	0.154	0.079
	0.149
0ng/ml	0.08	0.076	0
	0.071

We measured random samples of E0111Fi within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	29.3	0.7	2.5
Intra-assay	2	18	21.4	0.7	3.5
Intra-assay	3	18	7.5	0.4	5.1

> Protocol Booklet

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