Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0111Fi are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/ml | 2.16 | 2.119 | 2.044 |
| 2.078 | |||
| 24ng/ml | 1.05 | 1.075 | 1 |
| 1.101 | |||
| 12ng/ml | 0.622 | 0.647 | 0.571 |
| 0.671 | |||
| 6ng/ml | 0.3 | 0.306 | 0.231 |
| 0.313 | |||
| 3ng/ml | 0.16 | 0.154 | 0.079 |
| 0.149 | |||
| 0ng/ml | 0.08 | 0.076 | 0 |
| 0.071 |
Precision
We measured random samples of E0111Fi within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 29.3 | 0.7 | 2.5 |
| Intra-assay | 2 | 18 | 21.4 | 0.7 | 3.5 |
| Intra-assay | 3 | 18 | 7.5 | 0.4 | 5.1 |
Protocols
References
Customer reviews and Q&As
