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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1499Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
72ng/ml	2.083	2.145	2.081
	2.207
36ng/ml	1.425	1.376	1.313
	1.328
18ng/ml	0.775	0.842	0.779
	0.909
9ng/ml	0.42	0.477	0.414
	0.535
4.5ng/ml	0.311	0.268	0.204
	0.224
0ng/ml	0.059	0.064	0
	0.068

We measured random samples of E1499Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	27.1	1.4	5.3
Intra-assay	2	18	42.8	1.9	4.5
Intra-assay	3	18	11.2	0.6	5.7

> Protocol Booklet

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