Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1499Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 72ng/ml | 2.083 | 2.145 | 2.081 |
| 2.207 | |||
| 36ng/ml | 1.425 | 1.376 | 1.313 |
| 1.328 | |||
| 18ng/ml | 0.775 | 0.842 | 0.779 |
| 0.909 | |||
| 9ng/ml | 0.42 | 0.477 | 0.414 |
| 0.535 | |||
| 4.5ng/ml | 0.311 | 0.268 | 0.204 |
| 0.224 | |||
| 0ng/ml | 0.059 | 0.064 | 0 |
| 0.068 |
Precision
We measured random samples of E1499Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 27.1 | 1.4 | 5.3 |
| Intra-assay | 2 | 18 | 42.8 | 1.9 | 4.5 |
| Intra-assay | 3 | 18 | 11.2 | 0.6 | 5.7 |
Protocols
References
Customer reviews and Q&As
