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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0740Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.31	2.307	2.262
	2.303
60ng/ml	1.202	1.267	1.222
	1.332
30ng/ml	0.756	0.797	0.752
	0.837
15ng/ml	0.444	0.44	0.395
	0.435
7.5ng/ml	0.239	0.21	0.165
	0.181
0ng/ml	0.054	0.045	0
	0.035

We measured random samples of E0740Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	35.4	2	5.6
Intra-assay	2	18	63.4	2	3.2
Intra-assay	3	18	28.2	2.1	7.4

> Protocol Booklet

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