Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1285Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.604 | 2.482 | 2.428 |
| 2.359 | |||
| 600ng/L | 1.348 | 1.426 | 1.372 |
| 1.503 | |||
| 300ng/L | 0.86 | 0.886 | 0.833 |
| 0.912 | |||
| 150ng/L | 0.434 | 0.452 | 0.399 |
| 0.471 | |||
| 75ng/L | 0.285 | 0.259 | 0.206 |
| 0.233 | |||
| 0ng/L | 0.05 | 0.054 | 0 |
| 0.058 |
Precision
We measured random samples of E1285Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 303.5 | 16.7 | 5.5 |
| Intra-assay | 2 | 18 | 526.9 | 19.7 | 3.7 |
| Intra-assay | 3 | 18 | 120.9 | 6.9 | 5.7 |
Protocols
References
Customer reviews and Q&As
