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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1182Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ug/ml	2.268	2.253	2.202
	2.238
400ug/ml	1.37	1.378	1.328
	1.387
200ug/ml	0.82	0.823	0.773
	0.827
100ug/ml	0.465	0.423	0.372
	0.381
50ug/ml	0.206	0.221	0.17
	0.236
0ug/ml	0.047	0.051	0
	0.054

We measured random samples of E1182Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	418.1	17.3	4.1
Intra-assay	2	18	103.4	6.5	6.3
Intra-assay	3	18	254.6	12.2	4.8

> Protocol Booklet

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