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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0977Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.426	2.316	2.267
	2.206
600pg/ml	1.415	1.324	1.275
	1.233
300pg/ml	0.883	0.886	0.837
	0.89
150pg/ml	0.503	0.466	0.417
	0.43
75pg/ml	0.325	0.31	0.261
	0.295
0pg/ml	0.039	0.049	0
	0.059

We measured random samples of E0977Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	172	13.3	7.7
Intra-assay	2	18	726.8	24.8	3.4
Intra-assay	3	18	316.7	22.3	7

> Protocol Booklet

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