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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1987Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
192ng/ml	2.226	2.202	2.115
	2.177
96ng/ml	1.167	1.14	1.054
	1.113
48ng/ml	0.611	0.598	0.511
	0.585
24ng/ml	0.3	0.289	0.203
	0.278
12ng/ml	0.168	0.163	0.077
	0.158
0ng/ml	0.083	0.086	0
	0.089

We measured random samples of E1987Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	40.1	1.4	3.6
Intra-assay	2	18	86.9	3.3	3.8
Intra-assay	3	18	135.3	3.9	2.9

> Protocol Booklet

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