Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1987Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 192ng/ml | 2.226 | 2.202 | 2.115 |
| 2.177 | |||
| 96ng/ml | 1.167 | 1.14 | 1.054 |
| 1.113 | |||
| 48ng/ml | 0.611 | 0.598 | 0.511 |
| 0.585 | |||
| 24ng/ml | 0.3 | 0.289 | 0.203 |
| 0.278 | |||
| 12ng/ml | 0.168 | 0.163 | 0.077 |
| 0.158 | |||
| 0ng/ml | 0.083 | 0.086 | 0 |
| 0.089 |
Precision
We measured random samples of E1987Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 40.1 | 1.4 | 3.6 |
| Intra-assay | 2 | 18 | 86.9 | 3.3 | 3.8 |
| Intra-assay | 3 | 18 | 135.3 | 3.9 | 2.9 |
Protocols
References
Customer reviews and Q&As
