Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0504Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
8ng/ml | 2.072 | 1.978 | 1.934 |
1.884 | |||
4ng/ml | 0.934 | 0.971 | 0.928 |
1.008 | |||
2ng/ml | 0.598 | 0.54 | 0.497 |
0.483 | |||
1ng/ml | 0.193 | 0.183 | 0.139 |
0.173 | |||
0.5ng/ml | 0.111 | 0.111 | 0.067 |
0.11 | |||
0ng/ml | 0.032 | 0.044 | 0 |
0.055 |
Precision
We measured random samples of E0504Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 4.7 | 0.2 | 4.1 |
Intra-assay | 2 | 18 | 6 | 0.2 | 3.2 |
Intra-assay | 3 | 18 | 2.7 | 0.1 | 3.7 |
Protocols
References
Customer reviews and Q&As