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Home > Product > ELISA Kit > Human Phosphotylinosital 3 kinase, PI3K ELISA Kit

Human Phosphotylinosital 3 kinase, PI3K ELISA Kit

Human Phosphotylinosital 3 kinase, PI3K ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.13ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Phosphotylinosital 3 kinase

Code

E0896Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.13ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Research area

Signal Transduction;Cancer;Immunology;Neuroscience

Target protein

PI3K

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E0896Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 1.877 1.915 1.869
1.953
20ng/ml 1.085 1.095 1.049
1.104
10ng/ml 0.421 0.435 0.389
0.449
5ng/ml 0.265 0.222 0.176
0.179
2.5ng/ml 0.068 0.06 0.015
0.053
0ng/ml 0.034 0.046 0
0.057

Precision

We measured random samples of E0896Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 13.4 0.4 3.2
Intra-assay 2 18 33.7 1.5 4.5
Intra-assay 3 18 21.2 1 4.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E0896Hu

    $320.00

  • 96T

    E0896Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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