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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0788Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/mL	1.804	1.91	1.878
	2.016
16ng/mL	1.095	1.052	1.02
	1.008
8ng/mL	0.473	0.462	0.43
	0.451
4ng/mL	0.166	0.213	0.181
	0.26
2ng/mL	0.055	0.076	0.045
	0.098
0ng/mL	0.034	0.032	0
	0.03

We measured random samples of E0788Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	13.1	0.6	4.3
Intra-assay	2	18	24.5	0.7	2.8
Intra-assay	3	18	8.4	0.3	3.2

> Protocol Booklet

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