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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E4070Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.248	2.286	2.233
	2.324
600ng/L	1.345	1.333	1.28
	1.321
300ng/L	0.859	0.795	0.742
	0.732
150ng/L	0.389	0.445	0.392
	0.502
75ng/L	0.213	0.197	0.144
	0.181
0ng/L	0.06	0.053	0
	0.046

We measured random samples of E4070Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	269.3	17.7	6.6
Intra-assay	2	18	332	15.5	4.7
Intra-assay	3	18	756.7	31.6	4.2

> Protocol Booklet

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