Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0620Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
320pg/ml | 2.354 | 2.283 | 2.223 |
2.212 | |||
160pg/ml | 1.278 | 1.352 | 1.291 |
1.426 | |||
80pg/ml | 0.834 | 0.774 | 0.713 |
0.713 | |||
40pg/ml | 0.497 | 0.491 | 0.43 |
0.485 | |||
20pg/ml | 0.306 | 0.291 | 0.231 |
0.277 | |||
0pg/ml | 0.048 | 0.06 | 0 |
0.072 |
Precision
We measured random samples of E0620Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 48.6 | 2 | 4.1 |
Intra-assay | 2 | 18 | 81.5 | 4.2 | 5.2 |
Intra-assay | 3 | 18 | 218.6 | 7.8 | 3.6 |
Protocols
References
Customer reviews and Q&As