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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0211Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640pg/ml	2.347	2.246	2.215
	2.146
320pg/ml	1.368	1.363	1.332
	1.359
160pg/ml	0.696	0.73	0.699
	0.765
80pg/ml	0.381	0.42	0.388
	0.459
40pg/ml	0.199	0.198	0.166
	0.197
0pg/ml	0.037	0.032	0
	0.026

We measured random samples of E0211Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	266.2	15.8	5.9
Intra-assay	2	18	347.8	10.1	2.9
Intra-assay	3	18	125.8	7.2	5.7

> Protocol Booklet

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