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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E3394Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ug/mL	1.946	2	1.905
	2.054
6ug/mL	1.148	1.127	1.033
	1.106
3ug/mL	0.581	0.558	0.464
	0.536
1.5ug/mL	0.317	0.31	0.216
	0.303
0.75ug/mL	0.177	0.165	0.071
	0.153
0ug/mL	0.088	0.094	0
	0.101

We measured random samples of E3394Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	3.6	0.2	5.1
Intra-assay	2	18	9.4	0.3	2.7
Intra-assay	3	18	4.5	0.2	3.7

> Protocol Booklet

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