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Home > Product > ELISA Kit > Human Small Heterodimer Partner, SHP ELISA Kit

Human Small Heterodimer Partner, SHP ELISA Kit

Human Small Heterodimer Partner, SHP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.19ng/L

Detection range: 20-3800ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.19ng/L

Detection range: 20-3800ng/L

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Small Heterodimer Partner

Code

E6818Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.19ng/L

Detection range

20-3800ng/L

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with which it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1. Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism. Regulates the circadian expression of cytochrome P450 (CYP) enzymes.

Research area

Epigenetics and Nuclear Signaling|Signal Transduction

Target protein

SHP

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E6818Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.268 2.318 2.281
2.367
1000ng/L 1.886 1.817 1.78
1.748
500ng/L 1.111 1.214 1.177
1.317
250ng/L 0.604 0.64 0.603
0.676
125ng/L 0.362 0.375 0.338
0.389
0ng/L 0.037 0.037 0
0.037

Precision

We measured random samples of E6818Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 621 21.8 3.5
Intra-assay 2 18 204 9.8 4.8
Intra-assay 3 18 303.9 17.9 5.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6818Hu

    $320.00

  • 96T

    E6818Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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