Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Sirtuin 3, SIRT3 ELISA Kit

Human Sirtuin 3, SIRT3 ELISA Kit

Human Sirtuin 3, SIRT3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.051ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.051ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Reactive with: Human

Product overview

Full product name

Sirtuin 3

Code

E2559Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.051ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Species

Human

Storage

2-8°C

Assay time

1h 30m

Background

NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

UniProt accession

MASS(Da)

43,573

GeneID

23410

Synonyms

SIRT3;SIR2L3

Gene names

SIRT3

Research area

Epigenetics and Nuclear Signaling

Target protein

SIRT3

Components

Components Quantity
Standard Solution 0.5ml x1
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard Diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop Solution 6ml x1
Substrate Solution A 6ml x1
Substrate Solution B 6ml x1
Wash Buffer Concentrate (25x) 20ml x1
Biotinylated Antibody 1ml x1

Protocol data

These standard curves of E2559Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.062 2.003 1.961
1.945
12ng/ml 1.089 1.109 1.067
1.129
6ng/ml 0.45 0.467 0.425
0.484
3ng/ml 0.206 0.251 0.209
0.296
1.5ng/ml 0.11 0.117 0.075
0.123
0ng/ml 0.025 0.042 0
0.059

Precision

We measured random samples of E2559Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 8 0.5 5.7
Intra-assay 2 18 12.4 0.5 3.9
Intra-assay 3 18 15.7 0.5 3

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2559Hu

    $320.00

  • 96T

    E2559Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up