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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0168Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.103	2.028	1.978
	1.953
6ng/ml	1.018	1.058	1.009
	1.099
3ng/ml	0.418	0.466	0.416
	0.513
1.5ng/ml	0.266	0.283	0.233
	0.301
0.75ng/ml	0.073	0.081	0.031
	0.089
0ng/ml	0.041	0.05	0
	0.059

We measured random samples of E0168Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	7.1	0.3	4.4
Intra-assay	2	18	8.1	0.2	2.7
Intra-assay	3	18	3.3	0.2	4.7

> Protocol Booklet

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