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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1528Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.534	2.541	2.498
	2.547
600ng/L	1.544	1.447	1.404
	1.349
300ng/L	0.731	0.784	0.741
	0.837
150ng/L	0.528	0.515	0.472
	0.501
75ng/L	0.284	0.267	0.224
	0.249
0ng/L	0.043	0.042	0
	0.042

We measured random samples of E1528Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	723.7	20.3	2.8
Intra-assay	2	18	241.5	16.4	6.8
Intra-assay	3	18	343.1	20.2	5.9

> Protocol Booklet

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