Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E6666Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 32ng/ml | 1.992 | 1.989 | 1.967 |
| 1.987 | |||
| 16ng/ml | 1.544 | 1.549 | 1.527 |
| 1.555 | |||
| 8ng/ml | 0.712 | 0.7 | 0.677 |
| 0.687 | |||
| 4ng/ml | 0.42 | 0.369 | 0.347 |
| 0.318 | |||
| 2ng/ml | 0.116 | 0.12 | 0.097 |
| 0.123 | |||
| 0ng/ml | 0.023 | 0.022 | 0 |
| 0.021 |
Precision
We measured random samples of E6666Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 18.8 | 0.9 | 4.9 |
| Intra-assay | 2 | 18 | 6.4 | 0.2 | 3.4 |
| Intra-assay | 3 | 18 | 11.6 | 0.7 | 5.6 |
Protocols
References
Customer reviews and Q&As
