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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0025Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.033	2.197	2.154
	2.361
600pg/ml	1.614	1.567	1.523
	1.52
300pg/ml	1.047	1.047	1.004
	1.047
150pg/ml	0.632	0.686	0.643
	0.741
75pg/ml	0.444	0.437	0.394
	0.43
0pg/ml	0.055	0.043	0
	0.032

We measured random samples of E0025Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	166.5	11.1	6.7
Intra-assay	2	18	782.9	34.2	4.4
Intra-assay	3	18	103.4	7.7	7.4

> Protocol Booklet

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