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Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E1820Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400mg/L	2.106	2.214	2.161
	2.322
1200mg/L	1.852	1.782	1.729
	1.712
600mg/L	1.133	1.195	1.142
	1.257
300mg/L	0.725	0.692	0.639
	0.66
150mg/L	0.439	0.439	0.386
	0.44
0mg/L	0.047	0.053	0
	0.059

We measured random samples of E1820Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	1234.5	74.3	6
Intra-assay	2	18	270.1	14.5	5.4
Intra-assay	3	18	606.6	42.3	7

> Protocol Booklet

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