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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0921Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	1.984	2.019	1.965
	2.054
3.2ng/ml	1.009	0.975	0.921
	0.94
1.6ng/ml	0.455	0.502	0.448
	0.548
0.8ng/ml	0.268	0.224	0.17
	0.179
0.4ng/ml	0.118	0.132	0.078
	0.145
0ng/ml	0.056	0.054	0
	0.053

We measured random samples of E0921Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	3.5	0.1	4.3
Intra-assay	2	18	5.9	0.2	3.3
Intra-assay	3	18	2.4	0.1	4.5

> Protocol Booklet

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