Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Research area
Target protein
Components
Components | Quantity |
---|---|
Standard Solution | 0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (25x) | 20ml x1 |
Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0612Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
240nmol/ml | 2.094 | 2.156 | 2.109 |
2.217 | |||
120nmol/ml | 1.949 | 1.935 | 1.888 |
1.921 | |||
60nmol/ml | 1.131 | 1.129 | 1.082 |
1.127 | |||
30nmol/ml | 0.686 | 0.71 | 0.663 |
0.734 | |||
15nmol/ml | 0.401 | 0.411 | 0.364 |
0.42 | |||
0nmol/ml | 0.058 | 0.047 | 0 |
0.036 |
Precision
We measured random samples of E0612Ra within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 18 | 108.6 | 7.5 | 6.9 |
Intra-assay | 2 | 18 | 16.4 | 0.8 | 4.9 |
Intra-assay | 3 | 18 | 38.3 | 1.8 | 4.8 |
Protocols
References
Customer reviews and Q&As