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Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E3932Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
200ng/ml	1.885	1.943	1.904
	2.001
100ng/ml	0.967	1.038	0.999
	1.11
50ng/ml	0.403	0.463	0.424
	0.522
25ng/ml	0.182	0.235	0.196
	0.289
12.5ng/ml	0.157	0.145	0.106
	0.133
0ng/ml	0.049	0.039	0
	0.029

We measured random samples of E3932Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	155.1	4.8	3.1
Intra-assay	2	18	43.2	1.9	4.5
Intra-assay	3	18	87	4.4	5.1

> Protocol Booklet

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