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Home > Product > ELISA Kit > Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit

Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit

Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.32ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.32ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Indoleamine 2,3-Dioxygenase 1

Code

E2147Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.32ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity (PubMed: 25691885). Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3).

UniProt accession

MASS(Da)

45,641

GeneID

15930

Synonyms

IDO 1;IDO1;IDO-1;Indoleamine 2,3-dioxygenase 1;Indoleamine-pyrrole 2,3-dioxygenase

Gene names

IDO1

Research area

Metabolism

Target protein

IDO1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2147Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.181 2.201 2.146
2.221
2400ng/L 1.817 1.837 1.781
1.856
1200ng/L 1.132 1.175 1.119
1.217
600ng/L 0.752 0.735 0.68
0.718
300ng/L 0.428 0.436 0.381
0.444
0ng/L 0.062 0.055 0
0.049

Precision

We measured random samples of E2147Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 995.9 66.7 6.7
Intra-Assay 2 18 2004.5 119.76 6
Intra-Assay 3 18 401.4 19.44 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E2147Mo

    $320.00

  • 96T

    E2147Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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