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Home > Product > ELISA Kit > Mouse Interleukin-1 Receptor Accessory Protein, IL1RAP ELISA Kit

Mouse Interleukin-1 Receptor Accessory Protein, IL1RAP ELISA Kit

Mouse Interleukin-1 Receptor Accessory Protein, IL1RAP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 23.84ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 23.84ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interleukin-1 Receptor Accessory Protein

Code

E2172Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

23.84ng/L

Detection range

50-10000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (PubMed: 25908590). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity). [Isoform 2]: Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling. [Isoform 3]: Required for Src phosphorylation by IL1B. Required for IL1B-potentiated NMDA-induced calcium influx in neurons acting in cooperation with IL1R1 isoform 2 to mediate Akt kinase activation.

UniProt accession

MASS(Da)

65,741

GeneID

16180

Synonyms

IL 1RAP;IL-1 receptor accessory protein;IL-1RAcP;IL1RAP;Interleukin-1 receptor accessory protein;Interleukin-33 receptot beta chain

Gene names

IL1RAP

Research area

Immunology

Target protein

IL1RAP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2172Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6400ng/L 2.08 2.105 2.049
2.13
3200ng/L 1.242 1.313 1.257
1.384
1600ng/L 0.847 0.813 0.757
0.779
800ng/L 0.444 0.433 0.377
0.422
400ng/L 0.172 0.172 0.115
0.171
0ng/L 0.061 0.056 0
0.051

Precision

We measured random samples of E2172Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 795.4 40.58 5.1
Intra-Assay 2 18 3892.2 146.86 3.8
Intra-Assay 3 18 1645.1 91.33 5.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2172Mo

    $320.00

  • 96T

    E2172Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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