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Home > Product > ELISA Kit > Mouse Kelch-like Ech-associated Protein 1, KEAP1 ELISA Kit

Mouse Kelch-like Ech-associated Protein 1, KEAP1 ELISA Kit

Mouse Kelch-like Ech-associated Protein 1, KEAP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.97ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.97ng/L

Detection range: 2-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Kelch-Like Ech-Associated Protein 1

Code

E2198Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.97ng/L

Detection range

2-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed: 9887101, PubMed: 12682069, PubMed: 15282312, PubMed: 15367669, PubMed: 15581590). KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed: 9887101, PubMed: 12193649, PubMed: 14764894). In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed: 12193649, PubMed: 20$498.00|$360.00371, PubMed: 22014577, PubMed: 29590092). In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2 (PubMed: 20421418, PubMed: 20173742, PubMed: 24011591). The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (PubMed: 28380357). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (By similarity).

UniProt accession

MASS(Da)

69,553

GeneID

50868

Synonyms

Cytosolic inhibitor of Nrf2;INrf2;KEAP 1;KEAP1;Kelch-like ECH-associated protein 1

Gene names

KEAP1

Research area

Epigenetics and Nuclear Signaling

Target protein

KEAP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2198Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.31 2.266 2.215
2.221
160ng/L 1.773 1.849 1.798
1.925
80ng/L 1.197 1.198 1.147
1.198
40ng/L 0.745 0.676 0.625
0.607
20ng/L 0.369 0.372 0.321
0.375
0ng/L 0.04 0.051 0
0.061

Precision

We measured random samples of E2198Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 105.6 4.2 4
Intra-Assay 2 18 32.4 1.66 5.1
Intra-Assay 3 18 76 4.95 6.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E2198Mo

    $320.00

  • 96T

    E2198Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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