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Home > Product > ELISA Kit > Mouse Long-chain-Fatty-acid--CoA Ligase 1, ACSL1 ELISA Kit

Mouse Long-chain-Fatty-acid--CoA Ligase 1, ACSL1 ELISA Kit

Mouse Long-chain-Fatty-acid--CoA Ligase 1, ACSL1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.02ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.02ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Long-Chain-Fatty-Acid--Coa Ligase 1

Code

E2218Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.02ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (By similarity). Preferentially uses palmitoleate, oleate and linoleate (By similarity). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs).

UniProt accession

MASS(Da)

77,951

GeneID

14081

Synonyms

ACSL 1;ACSL1;Arachidonate--CoA ligase;LACS 1;Long-chain acyl-CoA synthetase 1;Long-chain-fatty-acid--CoA ligase 1;Phytanate--CoA ligase

Gene names

ACSL1

Research area

Signal Transduction

Target protein

ACSL1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2218Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.497 2.479 2.442
2.46
1000ng/L 1.483 1.445 1.408
1.406
500ng/L 0.774 0.831 0.794
0.888
250ng/L 0.45 0.414 0.377
0.377
125ng/L 0.203 0.225 0.188
0.247
0ng/L 0.043 0.037 0
0.031

Precision

We measured random samples of E2218Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 618.8 31.46 5.1
Intra-Assay 2 18 1056.8 29.54 2.8
Intra-Assay 3 18 341.3 17.86 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2218Mo

    $320.00

  • 96T

    E2218Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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