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Home > Product > ELISA Kit > Mouse Lysosomal Pro-X Carboxypeptidase, PRCP ELISA Kit

Mouse Lysosomal Pro-X Carboxypeptidase, PRCP ELISA Kit

Mouse Lysosomal Pro-X Carboxypeptidase, PRCP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.57ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.57ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Lysosomal Pro-X Carboxypeptidase

Code

E2225Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.57ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Cleaves C-terminal amino acids linked to proline in Peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity).

UniProt accession

MASS(Da)

55,027

GeneID

72461

Synonyms

Lysosomal Pro-X carboxypeptidase;PRCP;Proline carboxypeptidase;Prolylcarboxypeptidase

Gene names

PRCP

Research area

Cardiovascular

Target protein

PRCP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2225Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.184 2.219 2.178
2.253
1200ng/L 1.379 1.28 1.239
1.181
600ng/L 0.558 0.649 0.609
0.741
300ng/L 0.362 0.403 0.363
0.444
150ng/L 0.145 0.175 0.135
0.205
0ng/L 0.049 0.04 0
0.032

Precision

We measured random samples of E2225Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1747.9 56.84 3.3
Intra-Assay 2 18 422.8 21.7 5.1
Intra-Assay 3 18 918.9 47.63 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2225Mo

    $320.00

  • 96T

    E2225Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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