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Home > Product > ELISA Kit > Human Podoplanin, PDPN ELISA Kit

Human Podoplanin, PDPN ELISA Kit

Human Podoplanin, PDPN ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.05ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.05ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Podoplanin

Code

E0123Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.05ng/ml

Detection range

0.1-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed: 14522983, PubMed: 15231832, PubMed: 17616532, PubMed: 18215137, PubMed: 17222411). Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed: 18541721). Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed: 17046996, PubMed: 21376833). Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (PubMed: 20962267). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity). Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed: 19268462). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed: 15515019). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed: 25486435). Required for normal lung cell proliferation and alveolus formation at birth (By similarity). Does not function as a water Channel or as a regulator of aquaporin-type water Channels (PubMed: 9651190). Does not have any effect on folic acid or amino acid transport (By similarity).

UniProt accession

MASS(Da)

16,698

GeneID

10630

Synonyms

29kDa cytosolic podoplanin intracellular domain;Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;PDPN;PICD;Podoplanin;T1A;T1-alpha

Gene names

PDPN

Research area

Others

Target protein

PDPN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0123Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.09 2.059 1.998
2.029
8ng/ml 0.978 1.057 0.996
1.136
4ng/ml 0.595 0.502 0.441
0.408
2ng/ml 0.194 0.245 0.184
0.296
1ng/ml 0.161 0.118 0.057
0.076
0ng/ml 0.063 0.061 0
0.059

Precision

We measured random samples of E0123Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.3 0.31 2.7
Intra-Assay 2 18 9.2 0.44 4.8
Intra-Assay 3 18 4.5 0.2 4.3

Protocols

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  • Size

    Code

    Price

  • 96T

    E0123Hu

    $458.00

  • 48T

    E0123Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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