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Home > Product > ELISA Kit > Mouse Monocyte Differentiation Antigen Cd14, CD14 ELISA Kit

Mouse Monocyte Differentiation Antigen Cd14, CD14 ELISA Kit

Mouse Monocyte Differentiation Antigen Cd14, CD14 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Monocyte Differentiation Antigen Cd14

Code

E2267Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed: 16148141). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed: 8612135, PubMed: 15895089). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (By similarity). Acts as an accessory receptor for M.tuberculosis lipoproteins LprA, LprG and LpqH, in conjunction with coreceptors TLR2 and TLR1. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed: 19362712). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity).

UniProt accession

MASS(Da)

39,204

GeneID

12475

Synonyms

CD 14;CD antigen CD14;CD14;LPS-R;Mo2;Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein

Gene names

CD14

Research area

Immunology; Stem Cells

Target protein

CD14

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2267Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.082 2.236 2.197
2.389
6ng/mL 1.264 1.308 1.269
1.352
3ng/mL 0.754 0.703 0.664
0.651
1.5ng/mL 0.376 0.383 0.344
0.39
0.75ng/mL 0.18 0.172 0.133
0.165
0ng/mL 0.048 0.039 0
0.03

Precision

We measured random samples of E2267Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.8 0.2 5.3
Intra-Assay 2 18 7.2 0.22 3
Intra-Assay 3 18 2.6 0.13 4.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E2267Mo

    $320.00

  • 96T

    E2267Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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