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Home > Product > ELISA Kit > Mouse Nuclear Receptor Subfamily 4 Group A Member 3, NR4A3 ELISA Kit

Mouse Nuclear Receptor Subfamily 4 Group A Member 3, NR4A3 ELISA Kit

Mouse Nuclear Receptor Subfamily 4 Group A Member 3, NR4A3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.76ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.76ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Nuclear Receptor Subfamily 4 Group A Member 3

Code

E2314Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.76ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional activator that binds to regulatory elements in promoter regions in a cell- and response element (target)-specific manner (PubMed: 12709428). Induces gene expression by binding as monomers to the NR4A1 response element (NBRE) 5'-AAAAGGTCA-3' site and as homodimers to the Nur response element (NurRE) site in the promoter of their regulated target genes (By similarity). Plays a role in the regulation of proliferation, survival and differentiation of many different cell types and also in metabolism and inflammation. Mediates proliferation of vascular smooth muscle, myeloid progenitor cell and type B pancreatic cells; promotes mitogen-induced vascular smooth muscle cell proliferation through transactivation of SKP2 promoter by binding a NBRE site (PubMed: 21868379). Upon PDGF stimulation, stimulates vascular smooth muscle cell proliferation by regulating CCND1 and CCND2 expression. In islets, induces type B pancreatic cell proliferation through up-regulation of genes that activate cell cycle, as well as genes that cause degradation of the CDKN1A (By similarity). Negatively regulates myeloid progenitor cell proliferation by repressing RUNX1 in a NBRE site-independent manner (PubMed: 24806827). During inner ear, plays a role as a key mediator of the proliferative growth phase of semicircular canal development (PubMed: 11784868). Mediates also survival of neuron and smooth muscle cells; mediates CREB-induced neuronal survival, and during hippocampus development, plays a critical role in pyramidal cell survival and axonal guidance (PubMed: 20566846, PubMed: 15456880). Is required for S phase entry of the cell cycle and survival of smooth muscle cells by inducing CCND1, resulting in RB1 phosphorylation. Binds to NBRE motif in CCND1 promoter, resulting in the activation of the promoter and CCND1 transcription (PubMed: 19153266). Plays also a role in inflammation; upon TNF stimulation, mediates monocyte adhesion by inducing the expression of VCAM1 and ICAM1 by binding to the NBRE consensus site (PubMed: 20558821). In mast cells activated by Fc-epsilon receptor cross-linking, promotes the synthesis and release of cytokines but impairs events leading to degranulation (PubMed: 2$458.00|$300.006680). Plays also a role in metabolism; by modulating feeding behavior; and by playing a role in energy balance by inhibiting the glucocorticoid-induced orexigenic neuropeptides AGRP expression, at least in part by forming a complex with activated NR3C1 on the AGRP- glucocorticoid response element (GRE), and thus weakening the DNA binding activity of NR3C1 (PubMed: 23897430, PubMed: 19523439). Upon catecholamines stimulation, regulates gene expression that controls oxidative metabolism in skeletal muscle (PubMed: 18325999). Plays a role in glucose transport by regulating translocation of the SLC2A4 glucose transporter to the cell surface (By similarity). Finally, during gastrulation plays a crucial role in the formation of anterior mesoderm by controlling cell migration (PubMed: 13129926). Inhibits adipogenesis (PubMed: 189$458.00|$300.0012). Also participates in cardiac hypertrophy by activating PARP1 (By similarity).

UniProt accession

MASS(Da)

68,454

GeneID

18124

Synonyms

CHN;CSMF;MINOR;NR 4A3;NR4A3;Nuclear receptor subfamily 4 group A member 3;Orphan nuclear receptor TEC;Translocated in extraskeletal chondrosarcoma

Gene names

NR4A3

Research area

Neuroscience

Target protein

NR4A3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2314Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.129 2.15 2.11
2.171
800ng/L 1.621 1.644 1.604
1.666
400ng/L 1.108 1.043 1.003
0.979
200ng/L 0.774 0.708 0.668
0.643
100ng/L 0.432 0.419 0.38
0.407
0ng/L 0.043 0.04 0
0.037

Precision

We measured random samples of E2314Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1010.3 67.29 6.7
Intra-Assay 2 18 268.6 19.41 7.2
Intra-Assay 3 18 132 10.43 7.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E2314Mo

    $320.00

  • 96T

    E2314Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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