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Home > Product > ELISA Kit > Human Stem Cell Factor/Mast Cell Growth Factor, SCF/MGF ELISA Kit

Human Stem Cell Factor/Mast Cell Growth Factor, SCF/MGF ELISA Kit

Human Stem Cell Factor/Mast Cell Growth Factor, SCF/MGF ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.52pg/ml

Detection range: 5-1800pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 2.52pg/ml

Detection range: 5-1800pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Stem Cell Factor/Mast Cell Growth Factor

Code

E0135Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.52pg/ml

Detection range

5-1800pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

UniProt accession

MASS(Da)

30,899

GeneID

4254

Synonyms

c-Kit ligand;Kit ligand;KITLG;Mast cell growth factor;MGF;SCF;sKITLG;Soluble KIT ligand;Stem cell factor;

Gene names

KITLG

Research area

Immunology

Target protein

KITLG

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0135Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200pg/ml 2.067 1.964 1.926
1.861
600pg/ml 1.175 1.166 1.128
1.157
300pg/ml 0.519 0.46 0.422
0.401
150pg/ml 0.232 0.244 0.206
0.256
75pg/ml 0.088 0.101 0.062
0.113
0pg/ml 0.049 0.038 0
0.028

Precision

We measured random samples of E0135Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 284.2 9.9 3.5
Intra-Assay 2 18 1007.1 34.28 3.4
Intra-Assay 3 18 617.3 31.29 5.1

Protocols

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  • Size

    Code

    Price

  • 96T

    E0135Hu

    $458.00

  • 48T

    E0135Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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