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Home > Product > ELISA Kit > Mouse Secretory Phospholipase A2 Receptor, PLA2R1 ELISA Kit

Mouse Secretory Phospholipase A2 Receptor, PLA2R1 ELISA Kit

Mouse Secretory Phospholipase A2 Receptor, PLA2R1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Secretory Phospholipase A2 Receptor

Code

E2444Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipases sPLA2-IB/PLA2G1B, sPLA2-X/PLA2G10 and, with lower affinity, sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B and sPLA2-X/PLA2G10. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis.

UniProt accession

MASS(Da)

170,512

GeneID

18779

Synonyms

180 kDa secretory phospholipase A2 receptor;M-type receptor;PLA 2R1;PLA2R;PLA2-R;PLA2R1;Secretory phospholipase A2 receptor;Soluble PLA2R;Soluble PLA2-R;Soluble secretory phospholipase A2 receptor

Gene names

PLA2R1

Research area

Cardiovascular

Target protein

PLA2R1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2444Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.319 2.29 2.219
2.261
6ng/mL 1.241 1.263 1.192
1.285
3ng/mL 0.766 0.817 0.746
0.867
1.5ng/mL 0.429 0.47 0.399
0.511
0.75ng/mL 0.311 0.315 0.244
0.318
0ng/mL 0.075 0.071 0
0.067

Precision

We measured random samples of E2444Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.8 0.19 6.6
Intra-Assay 2 18 6.4 0.19 3
Intra-Assay 3 18 4 0.23 5.6

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2444Mo

    $320.00

  • 96T

    E2444Mo

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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