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Home > Product > ELISA Kit > Mouse T-cell Surface Glycoprotein CD3 Zeta Chain, CD247 ELISA Kit

Mouse T-cell Surface Glycoprotein CD3 Zeta Chain, CD247 ELISA Kit

Mouse T-cell Surface Glycoprotein CD3 Zeta Chain, CD247 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.31ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 14.31ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

T-Cell Surface Glycoprotein Cd3 Zeta Chain

Code

E2506Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.31ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN).

UniProt accession

MASS(Da)

18,637

GeneID

12503

Synonyms

CD 247;CD antigen CD247;CD247;T-cell receptor T3 zeta chain;T-cell surface glycoprotein CD3 zeta chain

Gene names

CD247

Research area

Immunology

Target protein

CD247

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2506Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 1.756 1.822 1.781
1.888
2400ng/L 1.144 1.131 1.091
1.119
1200ng/L 0.456 0.543 0.503
0.63
600ng/L 0.152 0.168 0.128
0.184
300ng/L 0.151 0.131 0.09
0.111
0ng/L 0.046 0.04 0
0.035

Precision

We measured random samples of E2506Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4706.1 271.31 5.8
Intra-Assay 2 18 1551.9 64.99 4.2
Intra-Assay 3 18 2079.7 71.91 3.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E2506Mo

    $320.00

  • 96T

    E2506Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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