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Home > Product > ELISA Kit > Mouse Toll-like Receptor 8, TLR8 ELISA Kit

Mouse Toll-like Receptor 8, TLR8 ELISA Kit

Mouse Toll-like Receptor 8, TLR8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.55ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.55ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Toll-Like Receptor 8

Code

E2516Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.55ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce proinflammatory cytokines and interferons, respectively.

UniProt accession

MASS(Da)

119,358

GeneID

170744

Synonyms

CD antigen CD288;TLR 8;TLR8;Toll-like receptor 8

Gene names

TLR8

Research area

Immunology; Microbiology

Target protein

TLR8

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2516Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.118 2.196 2.123
2.274
240ng/L 1.226 1.241 1.168
1.255
120ng/L 0.748 0.742 0.669
0.736
60ng/L 0.546 0.514 0.441
0.482
30ng/L 0.321 0.311 0.237
0.301
0ng/L 0.076 0.073 0
0.07

Precision

We measured random samples of E2516Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 80.9 6.01 7.4
Intra-Assay 2 18 210.5 11.83 5.6
Intra-Assay 3 18 369.6 13.91 3.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E2516Mo

    $320.00

  • 96T

    E2516Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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