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Home > Product > ELISA Kit > Mouse Tsukushin, TSKU ELISA Kit

Mouse Tsukushin, TSKU ELISA Kit

Mouse Tsukushin, TSKU ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.055ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tsukushin

Code

E2530Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.055ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Contributes to various developmental events and other processes such as wound healing and cholesterol homeostasis through its interactions with multiple signaling pathways (PubMed: 21856951, PubMed: 22995554, PubMed: 25159578, PubMed: 31391339). Wnt signaling inhibitor which competes with WNT2B for binding to Wnt receptor FZD4 and represses WNT2B-dependent development of the peripheral eye (PubMed: 21856951). Plays a role in regulating the hair cycle by controlling TGFB1 signaling (PubMed: 22995554). Required for the development of the anterior commissure in the brain by inhibiting neurite outgrowth (PubMed: 21055390, PubMed: 23206892). Essential for terminal differentiation of hippocampal neural stem cells (PubMed: 31983064). Plays a role in regulating bone elongation and bone mass by modulating growth plate chondrocyte function and overall body size (PubMed: 30271858). Required for development of the inner ear through its involvement in stereocilia formation in inner hair cells (PubMed: 32127020). Facilitates wound healing by inhibiting secretion of TGFB1 from macrophages which prevents myofibroblast differentiation, maintaining inflammatory cell quiescence (PubMed: 25159578). Plays a role in cholesterol homeostasis by reducing circulating high-density lipoprotein cholesterol, lowering cholesterol efflux capacity and decreasing cholesterol-to-bile acid conversion in the liver (PubMed: 31391339). In one study, shown to negatively regulate sympathetic innervation in brown fat, leading to reduced energy expenditure (PubMed: 31535079). In another study, shown not to affect brown fat thermogenic capacity, body weight gain or glucose homeostasis (PubMed: 31767170).

UniProt accession

MASS(Da)

38,362

GeneID

244152

Synonyms

Leucine-rich repeat-containing protein 54;TSKU;Tsukushi;Tsukushin

Gene names

TSKU

Research area

Signal Transduction

Target protein

TSKU

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2530Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.046 2.208 2.171
2.371
12ng/mL 1.491 1.618 1.582
1.746
6ng/mL 1.127 1.115 1.078
1.102
3ng/mL 0.648 0.666 0.629
0.684
1.5ng/mL 0.428 0.449 0.412
0.469
0ng/mL 0.041 0.037 0
0.032

Precision

We measured random samples of E2530Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.4 0.21 6.2
Intra-Assay 2 18 14.1 0.83 5.9
Intra-Assay 3 18 2.1 0.15 7.3

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2530Mo

    $320.00

  • 96T

    E2530Mo

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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