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Home > Product > ELISA Kit > Mouse Tyrosine-protein Phosphatase Non-receptor Type Substrate 1, SIRPA ELISA Kit

Mouse Tyrosine-protein Phosphatase Non-receptor Type Substrate 1, SIRPA ELISA Kit

Mouse Tyrosine-protein Phosphatase Non-receptor Type Substrate 1, SIRPA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.84ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.84ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1

Code

E2549Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.84ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity).

UniProt accession

MASS(Da)

56,414

GeneID

19261

Synonyms

Bit;Brain Ig-like molecule with tyrosine-based activation motifs;CD antigen CD172a;CD172 antigen-like family member A;Inhibitory receptor SHPS-1;mSIRP-alpha1;MyD-1 antigen;p84;SHP substrate 1;SHPS-1;Signal-regulatory protein alpha-1;SIRPA;Sirp-alpha-1;Tyrosine-protein phosphatase non-receptor type substrate 1

Gene names

SIRPA

Research area

Neuroscience

Target protein

SIRPA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2549Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.037 1.93 1.891
1.824
1200ng/L 0.952 1.052 1.012
1.152
600ng/L 0.576 0.512 0.473
0.449
300ng/L 0.19 0.223 0.183
0.255
150ng/L 0.088 0.096 0.057
0.105
0ng/L 0.033 0.04 0
0.046

Precision

We measured random samples of E2549Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1705.7 45.45 2.7
Intra-Assay 2 18 1255 39.03 3.1
Intra-Assay 3 18 749.1 39.1 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2549Mo

    $320.00

  • 96T

    E2549Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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