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Home > Product > ELISA Kit > Bovine Apelin, AP ELISA Kit

Bovine Apelin, AP ELISA Kit

Bovine Apelin, AP ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 2.47ng/ml

Detection range: 5-2000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 2.47ng/ml

Detection range: 5-2000ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Apelin

Code

E0168Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

2.47ng/ml

Detection range

5-2000ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Endogenous ligand for the apelin receptor (APLNR) (PubMed: 9792798). Drives internalization of APLNR (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).

UniProt accession

MASS(Da)

8,743

GeneID

282143

Synonyms

Apelin;Apelin-13;Apelin-28;Apelin-31;Apelin-36;APJ endogenous ligand;APLN

Gene names

APLN

Research area

Neuroscience

Target protein

APLN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0168Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/ml 2.163 2.18 2.143
2.198
600ng/ml 1.947 1.924 1.887
1.902
300ng/ml 1.133 1.19 1.153
1.248
150ng/ml 0.704 0.666 0.629
0.628
75ng/ml 0.413 0.4 0.363
0.388
0ng/ml 0.047 0.037 0
0.028

Precision

We measured random samples of E0168Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 397.6 17.68 4.4
Intra-Assay 2 18 97.1 5.67 5.8
Intra-Assay 3 18 286.1 15.21 5.3

Protocols

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  • Size

    Code

    Price

  • 96T

    E0168Bo

    $458.00

  • 48T

    E0168Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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