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Home > Product > ELISA Kit > Mouse Nad-dependent Protein Deacetylase Sirtuin-6, SIRT6 ELISA Kit

Mouse Nad-dependent Protein Deacetylase Sirtuin-6, SIRT6 ELISA Kit

Mouse Nad-dependent Protein Deacetylase Sirtuin-6, SIRT6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.68ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.68ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Nad-Dependent Protein Deacetylase Sirtuin-6

Code

E2587Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.68ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate (By similarity). Acts as a corepressor of the transcription factor Hif1a to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. Regulates the production of TNF protein. Has a role in the regulation of life span in male mice, but not in female mice.

UniProt accession

MASS(Da)

36,920

GeneID

50721

Synonyms

NAD-dependent protein deacetylase sirtuin-6;Regulatory protein SIR2 homolog 6;SIR2-like protein 6;SIRT 6;SIRT6

Gene names

SIRT6

Research area

Epigenetics and Nuclear Signaling

Target protein

SIRT6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2587Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.213 2.132 2.081
2.052
400ng/L 1.371 1.399 1.348
1.428
200ng/L 0.8 0.842 0.79
0.884
100ng/L 0.535 0.467 0.415
0.399
50ng/L 0.31 0.311 0.26
0.313
0ng/L 0.033 0.052 0
0.07

Precision

We measured random samples of E2587Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 344.7 11.46 3.3
Intra-Assay 2 18 247.6 11.94 4.8
Intra-Assay 3 18 87 5.54 6.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E2587Mo

    $320.00

  • 96T

    E2587Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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