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Home > Product > ELISA Kit > Mouse Programmed Cell Death Protein 1, PDCD1 ELISA Kit

Mouse Programmed Cell Death Protein 1, PDCD1 ELISA Kit

Mouse Programmed Cell Death Protein 1, PDCD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.36ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.36ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Programmed Cell Death Protein 1

Code

E2590Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.36ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed: 10485649, PubMed: 11698646, PubMed: 11209085, PubMed: 21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed: 11015443, PubMed: 11224527, PubMed: 22641383, PubMed: 18287011, PubMed: 18641123). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed: 22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed: 11698646, PubMed: 22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).

UniProt accession

MASS(Da)

31,842

GeneID

18566

Synonyms

CD antigen CD279;mPD-1;PDCD 1;PDCD1;Programmed cell death protein 1;Protein PD-1

Gene names

PDCD1

Research area

Cell Biology

Target protein

PDCD1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2590Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.244 2.339 2.301
2.434
600ng/L 1.216 1.278 1.24
1.34
300ng/L 0.599 0.646 0.609
0.694
150ng/L 0.44 0.366 0.329
0.292
75ng/L 0.188 0.177 0.14
0.167
0ng/L 0.033 0.037 0
0.042

Precision

We measured random samples of E2590Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 340.6 19.33 5.7
Intra-Assay 2 18 694 18.54 2.7
Intra-Assay 3 18 533.9 28.19 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E2590Mo

    $320.00

  • 96T

    E2590Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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