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Home > Product > ELISA Kit > Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit

Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit

Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 2, SRD5A2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.33ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 3.33ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

3-Oxo-5-Alpha-Steroid 4-Dehydrogenase 2

Code

E2624Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.33ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

UniProt accession

MASS(Da)

28,619

GeneID

94224

Synonyms

3-oxo-5-alpha-steroid 4-dehydrogenase 2;5 alpha-SR2;S5AR 2;SR type 2;SRD5A2;Steroid 5-alpha-reductase 2

Gene names

SRD5A2

Research area

Signal Transduction

Target protein

SRD5A2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2624Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.335 2.248 2.201
2.161
400ng/L 1.445 1.461 1.414
1.476
200ng/L 0.853 0.862 0.815
0.872
100ng/L 0.434 0.491 0.444
0.548
50ng/L 0.247 0.278 0.231
0.309
0ng/L 0.061 0.047 0
0.033

Precision

We measured random samples of E2624Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 352.9 14.82 4.2
Intra-Assay 2 18 108 5.91 5.5
Intra-Assay 3 18 224.6 11.49 5.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2624Mo

    $320.00

  • 96T

    E2624Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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